Flow Cytometric Measurement of Reactive Oxygen Species to Assess the Effects of Preconditioning Total Body Irradiation on NOG Mice

• DOI: 10.31557/apjcp.2022.23.2.383
• Publication Date: 2022-02-28T15:01:22Z
• Authors: Maria Kavianpour; Kobra Moradzadeh; Samad Muhammadnejad; Zahra Jabbarpour; Amir Arsalan Khor...; Sajjad Aghayan; Mohammad Vasei; Javad Verdi
• Keywords: 0303 health sciences; Short Communication; Hematopoietic Stem Cell Transplantation; Flow Cytometry; 3. Good health; Mice; 03 medical and health sciences; Bone Marrow; Animals; Annexin A5; Reactive Oxygen Species; Whole-Body Irradiation; Propidium

Preclinical development of new drugs for cancer immunotherapy requires preconditioning total body irradiation (TBI) of mice to be humanized via hematopoietic stem cell transplantation. To assess the effect of preconditioning TBI, we detected the reactive oxygen species (ROS), Annexin V, propidium iodide (PI) level in bone marrow samples by flow cytometer.We divided all NOG mice between irradiated (n = 20) and control groups (n = 10) for two time points. Irradiated mice were exposed to 3.5 Gy of radiation. After sacrificing BM samples were collected, the flow cytometric percentage of ROS, Annexin V, and PI markers were investigated on days 2 and 14 after exposure.At the first time point, the level of ROS was higher in the irradiated group than in the control group, and this difference was statistically significant (P < 0.05). Also, at the second time point, the mean differences of all markers in the irradiated group were significantly compared to the control group (P < 0.05).Thus, in NOG mice, the measurement of ROS level is helpful to the assessment of preconditioning TBI.