Clinical Performance of Self-collected Specimen HPV-DNA vs Clinician- collected Specimen HPV-mRNA to Detect High-risk HPV and High-grade Cervical Lesions and Cancer

• DOI: 10.31557/apjcp.2024.25.1.211
• Publication Date: 2024-01-29T19:07:34Z
• Authors: Thannaporn Kittisiam; Saranya Chanpanit...; Siriwan Tangjitgamol; Sunamchok Srijaip...; Sumonmal Manusiri...; Jatupol Srisomboon; Wichai Termrungru...
• Keywords: Concordance

Objective: Self- collected specimens to detect high-risk (hr) HPV and high-grade cervical lesions (CIN2+) has been introduced aiming increase cancer screening coverage. The performance of self- specimen compared clinician is one major concern. This study aimed compare self-sampling HPV-DNA clinician-sampling HPV-mRNA hr-HPV lesions. Methods: Women with abnormal cytology and/ or positive who attended the colposcopy clinics in 10 tertiary hospitals Bangkok were enrolled. Self-collected evaluated for DNA using Cobas® 4800 test prior clinician-collected which tested mRNA APTIMA® Assay. Subsequent biopsy was performed. detection rates from both tests their pathology compared. Results: Data 497 women’s analyzed. Both samplings had 86.8% concordance rate detecting (Kappa 0.670; 95% confidence interval [CI] 0.599-0.746, P value < 0.001). sensitivity (95% CI) self-collected clinician- 91.8% (85.4%-96.0%) 90.2% (83.6%-94.9%) respectively. corresponding negative predictive values 91.9% (85.6%-96.0%) 91.7% (86.0%-95.7%) Conclusion: testing HR-HPV demonstrates high specimen. are comparable.