Combination of Click Chemistry and Enzymatic Ligation for Stable and Efficient Protein Immobilization for Single-Molecule Force Spectroscopy

• DOI: 10.31635/ccschem.021.202100779
• Publication Date: 2021-02-17T15:24:17Z
• Authors: Shengchao Shi; Ziyi Wang; Yibing Deng; Fang Tian; Qingsong Wu; Peng Zheng
• Keywords: 02 engineering and technology; 0210 nano-technology

Protein immobilization is an essential method for both basic and applied research for protein, and covalent, site-specific attachment is the most desirable strategy. Classic methods typically rely ...