Differences in uterine and serum metabolome associated with metritis in dairy cows

• DOI: 10.3168/jds.2022-22552
• Publication Date: 2023-03-07T22:14:27Z
• Authors: C.C. Figueiredo; L. Balzano-Nogueira; D.Z. Bisinotto; A. Revilla Ruiz; G.A. Duarte; A. Conesa; K.N. Galvão; R.S. Bisinotto
• Keywords: Metritis; Metabolome; Endometritis; Vaginal discharge; Ceftiofur

Objectives were to evaluate differences in the uterine and serum metabolomes associated with metritis dairy cows. Vaginal discharge was evaluated using a Metricheck device (Simcro) at 5, 7, 11 d milk (DIM; herd 1) or 4, 6, 8, 10, 12 DIM (herd 2). Cows reddish brownish, watery, fetid diagnosed (n = 24). paired herdmates without (i.e., clear mucous vaginal lochia ≤50% of pus) based on parity Day diagnosis considered study 0. All cows received antimicrobial therapy. The metabolome lavage collected 0 samples untargeted gas chromatography time-of-flight mass spectrometry. Normalized data subjected multivariate canonical analysis population MultBiplotR MixOmics packages R Studio. Univariate analyses including t-test, principal component analyses, partial least squares discriminant pathway conducted Metaboanalyst. differed between Differences related metabolism butanoate, amino acids glycine, serine, threonine, alanine, aspartate, glutamate), glycolysis gluconeogenesis, tricarboxylic acid cycle. No observed counterparts Similarly, no not 5. These results indicate that establishment is local disturbances acid, lipid, carbohydrate uterus. lack 5 indicates processes implicated disease are reestablished by after treatment.