Effect of intramammary infection and inflammation on milk protein profile assessed at the quarter level in Holstein cows

• DOI: 10.3168/jds.2023-23818
• Publication Date: 2023-10-19T03:42:38Z
• Authors: V. Bisutti; A. Vanzin; S. Pegolo; A. Toscano; M. Gianesella; E. Sturaro; S. Schiavon; L. Gallo; F. Tagliapietra; D. Giannuzzi; A. Cecchinato
• Keywords: Lactoferrin; Streptococcus uberis; Streptococcus dysgalactiae

In this study we wanted to investigate the associations between naturally occurring subclinical intramammary infection (IMI) caused by different etiological agents (i.e., Staphylococcus aureus, Streptococcus agalactiae, uberis, and Prototheca spp.), in combination with somatic cell count (SCC), on detailed milk protein profile measured at individual mammary gland quarter. An initial bacteriological screening (time 0; T0) conducted composite from 450 Holstein cows reared 3 herds, was performed identify IMI. We identified 78 infected animals which were followed up quarter level 2 sampling times: T1 T2, 6 wk after T0, respectively. A total of 529 samples belonging previously selected collected points analyzed a reversed phase HPLC (RP-HPLC) validated method. Specifically, quantified 4 caseins (CN), namely α