Co-assembled whey protein and proanthocyanidins as a promising biocarrier for hydrophobic pterostilbene: Fabrication, characterization, and cellular antioxidant potential

• DOI: 10.3168/jds.2023-23925
• Publication Date: 2023-11-09T08:20:42Z
• Authors: Weigang Zhong; Qi Wang; Min Li; Xuming Deng; Xue Shen
• Keywords: ABTS; Whey protein isolate; Zeta potential; Hydrophobic effect; Pterostilbene; Random coil

The usage of food-derived polyphenols with different polarities has been limited by their instability and incompatibility. Therefore, a biocarrier was developed co-assembly whey protein isolate (WPI) hydrophilic proanthocyanidin (PC) for loading hydrophobic pterostilbene (PTE). Such superior affinity PTE than WPI alone, as determined encapsulation efficiency capacity assay, fluorescence quenching analysis, molecular docking, whereas the assembly process characterized particle size zeta potential, 3-dimensional fluorescence, scanning electron microscopy. Circular dichroism Fourier transform infrared spectroscopy spectra confirmed α-helix to β-sheet random coil transition proteins during formation nanocomplexes. Whey acted mediator through altering binding mode PC PTE, allowing them perform significant synergistic effects in enhancing 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 2,2-diphenyl-1-picrylhydrazyl radical scavenging reducing H