Preventive effects of lactoferrin on acute alcohol-induced liver injury via iron chelation and regulation of iron metabolism

• DOI: 10.3168/jds.2023-24490
• Publication Date: 2024-04-11T00:49:14Z
• Authors: Shuang Guan; Shengzhuo Zhang; Meitong Liu; Jiakang Guo; Yuelin Chen; Xue Shen; Xuming Deng; Jing Lu
• Keywords: Lactoferrin

Lactoferrin is widely found in milk and has the ability to bind iron. Previous studies have reported that lactoferrin was effective prevention treatment of acute alcohol-induced liver injury (AALI). Ferroptosis a recently discovered cell death involved development AALI. However, potential role ferroptosis still unclear. In this study, we observed (10, 20, 40 μg/mL) significantly mitigated alcohol (300 mM)-induced vitro. Additionally, (100 200 mg/kg BW) alleviated (4.8 g/kg BW)-induced vivo. Our results showed inhibited upregulation marker protein ACSL4 downregulation GPX4. Meanwhile, successfully reversed elevated malondialdehyde (MDA) levels reduced glutathione (GSH) caused by treatment. These may indicate decreased vivo chelate iron, our (20 iron ions expression ferritin heavy chain (FTH) under FeCl