Downregulation of serum microRNA-205 as a potential diagnostic and prognostic biomarker for human glioma

• DOI: 10.3171/2015.1.jns141577
• Publication Date: 2015-07-31T13:59:54Z
• Authors: Xiao Yue; FengMing Lan; Man Hu; Qiang Pan; Qiong Wang; JinHuan Wang
• Keywords: Adult; Male; Brain Neoplasms; Down-Regulation; Reproducibility of Results; Glioma; Kaplan-Meier Estimate; Middle Aged; Prognosis; Survival Analysis; 3. Good health; Cohort Studies; MicroRNAs; 03 medical and health sciences; 0302 clinical medicine; Risk Factors; Biomarkers, Tumor; Humans; Female; Lymphoma, Large B-Cell, Diffuse; Neoplasm Recurrence, Local; Meningioma; Aged

OBJECT Circulating microRNAs (miRNAs) are a new class of highly promising cancer biomarkers. Malignant glioma is one the most devastating and lethal forms intrinsic CNS tumor. Here, authors evaluated serum miRNA 205 (miR-205) levels in patients with glioma. METHODS Sixty-four whom was diagnosed 45 healthy controls were recruited between October 2011 March 2012 randomly assigned to screening cohort or validation cohort. Cohorts other brain tumors, including meningioma (n = 8), primary diffuse large B-cell lymphoma 6), pituitary adenoma 5), investigated compared. miR-205 extraction from detected by real-time quantitative reverse-transcription polymerase chain reaction. The Kaplan-Meier method applied perform survival analysis, risk factors analyzed using Cox regression model, receiver operating characteristic working curve used analyze value prognostic evaluation patients. RESULTS first demonstrated that expression significantly lower than (p < 0.001). It important note stepwise decrease ascending pathological grades. biomarker had high sensitivity, specificity, accuracy Serum identified as an individual diagnostic marker tumor cohorts. increased postoperative samples over those preoperative reduced again during glioblastoma recurrences. Statistical analysis revealed significant correlation low both grades 0.002) Karnofsky Performance Scale scores 0.01). Patients at advanced grade (Grade III IV) higher level showed longer overall concentration Furthermore, independently associated survival. CONCLUSIONS These data indicate novel valuable for diagnosis factor grade.