Effects of Poly(ADP-Ribose) Polymerase Inhibition in Bladder Damage Caused by Cyclophosphamide in Rats

• DOI: 10.3181/0706-rm-151
• Publication Date: 2008-05-12T23:11:21Z
• Authors: Ahmet Korkmaz; Bülent Kurt; Ibrahim Yildirim; Seref Basal; Turgut Topal; Serdar Sadir; Sükrü Öter
• Keywords: Ebselen; Pathogenesis; PARP inhibitor

It was previously shown that nitric oxide produced by inducible synthase (iNOS) and peroxynitrite are responsible for cyclophosphamide (CP)-induced cystitis. Since endogenous production of is known to lead poly(ADP-ribose) polymerase (PARP) activation, in this study, the aim evaluate whether PARP activation pathway also included pathogenesis CP-induced bladder ulceration rats. A total 48 male albino Wistar rats were divided into 5 groups. Group 1 served as control given 2 ml saline; four groups received a single dose CP (200 mg/kg) with same time intervals. only; 3, selective iNOS inhibitor 1400W (20 mg/kg); 4, scavenger ebselen (30 5, 3-aminobenzamide mg/kg). injection resulted severe cystitis continuous macroscopic hemorrhage, strong edema, inflammation, ulceration. Moreover, urine nitrite-nitrate levels dramatically increased. Histologically, protected against damage decreased induction. Ebselen has similar histologic results without changing urinary level activity. Furthermore group, beneficial effects had occurred including These suggest involves Furthermore, not only important but inflammation because broken uroepithelial cellular integrity.