Norovirus Recombination in ORF1/ORF2 Overlap

• DOI: 10.3201/eid1107.041273
• Publication Date: 2012-03-19T13:29:04Z
• Authors: Rowena A. Bull; Grant S. Hansman; Leighton E. Clancy; Mark M. Tanaka; William D. Rawlinson; Peter A. White
• Keywords: Subgenomic mRNA; Recombinant virus

Norovirus (NoV) genogroups I and II (GI GII) are now recognized as the predominant worldwide cause of outbreaks acute gastroenteritis in humans. Three recombinant NoV GII isolates were identified characterized, 2 which unrelated to any previously published NoV. Using data from current study, sequences, database searches, molecular techniques, we 23 1 GI isolates. Analysis genetic relationships among 9 independent sequences; other 14 strains close relatives. Two closely related recombinants only polymerase region, a similar fashion was another capsid region. Breakpoint analysis showed that recombination occurred open reading frame (ORF)1/ORF2 overlap. We provide evidence support theory role subgenomic RNA promoters hotspots describe simple mechanism how might occur