Co-infections of Adenovirus Species in Previously Vaccinated Patients
0301 basic medicine
Enzyme-Linked Immunosorbent Assay
Infectious and parasitic diseases
RC109-216
Polymerase Chain Reaction
Disease Outbreaks
molecular diagnostics
Adenovirus Infections, Human
03 medical and health sciences
respiratory infection
Adenovirus
Humans
Serotyping
Respiratory Tract Infections
Oligonucleotide Array Sequence Analysis
research
Research
Adenoviruses, Human
R
Viral Vaccines
coinfection
United States
3. Good health
Military Personnel
DNA, Viral
Medicine
microarray
DOI:
10.3201/eid1206.050245
Publication Date:
2011-12-19T19:52:28Z
AUTHORS (14)
ABSTRACT
Despite the success of the adenovirus vaccine administered to US military trainees, acute respiratory disease (ARD) surveillance still detected breakthrough infections (respiratory illnesses associated with the adenovirus serotypes specifically targeted by the vaccine). To explore the role of adenoviral co-infection (simultaneous infection by multiple pathogenic adenovirus species) in breakthrough disease, we examined specimens from patients with ARD by using 3 methods to detect multiple adenoviral species: a DNA microarray, a polymerase chain reaction (PCR)-enzyme-linked immunosorbent assay, and a multiplex PCR assay. Analysis of 52 samples (21 vaccinated, 31 unvaccinated) collected from 1996 to 2000 showed that all vaccinated samples had co-infections. Most of these co-infections were community-acquired serotypes of species B1 and E. Unvaccinated samples primarily contained only 1 species (species E) associated with adult respiratory illness. This study highlights the rarely reported phenomenon of adenoviral co-infections in a clinically relevant environment suitable for the generation of new recombinational variants.
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