Clostridium difficile PCR Ribotypes in Calves, Canada
2. Zero hunger
0303 health sciences
Clostridioides difficile
Research
R
Cryptosporidium
Clostridium difficile
Infectious and parasitic diseases
RC109-216
Microbial Sensitivity Tests
Calf diarrhea
Polymerase Chain Reaction
Ribotyping
3. Good health
03 medical and health sciences
cattle
Drug Resistance, Bacterial
Medicine
Animals
Cattle
fluoroquinolones
humans
Fluoroquinolones
DOI:
10.3201/eid1211.051581
Publication Date:
2011-11-02T13:53:39Z
AUTHORS (8)
ABSTRACT
We investigated Clostridium difficile in calves and the similarity between bovine and human C. difficile PCR ribotypes by conducting a case-control study of calves from 102 dairy farms in Canada. Fecal samples from 144 calves with diarrhea and 134 control calves were cultured for C. difficile and tested with an ELISA for C. difficile toxins A and B. C. difficile was isolated from 31 of 278 calves: 11 (7.6%) of 144 with diarrhea and 20 (14.9%) of 134 controls (p = 0.009). Toxins were detected in calf feces from 58 (56.8%) of 102 farms, 57 (39.6%) of 144 calves with diarrhea, and 28 (20.9%) of 134 controls (p = 0.0002). PCR ribotyping of 31 isolates showed 8 distinct patterns; 7 have been identified in humans, 2 of which have been associated with outbreaks of severe disease (PCR types 017 and 027). C. difficile may be associated with calf diarrhea, and cattle may be reservoirs of C. difficile for humans.
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