Experimental Infection and Response to Rechallenge of Alpacas with Middle East Respiratory Syndrome Coronavirus

• DOI: 10.3201/eid2206.160007
• Publication Date: 2016-03-03T01:19:26Z
• Authors: Gary Crameri; Peter A. Durr; Reuben Klein; Adam Foord; Meng Yu; Sarah Riddell; Jessica Haining; Dayna Johnson; Maged G. Hemida; Jennifer Barr; Malik Peiris; Deborah Middleton; Lin-Fa Wang
• Keywords: camel; coronavirus; Infectious and parasitic diseases; RC109-216; Infectious disease (medical specialty); FOS: Health sciences; Antibodies, Viral; immunology; Agricultural and Biological Sciences; MERS-CoV; animal; Disease; Viral; Viridae; Neutralizing; Internal medicine; serodiagnosis; 0303 health sciences; adult; Middle East respiratory syndrome; biotic associations; corona viruses; R; Dispatch; neutralizing antibody; Life Sciences; covid; New World; Viral Load; nose smear; New World camelid; virology; Virus Shedding; Virus; 3. Good health; female; Infectious Diseases; covid-19; real time polymerase chain reaction; virus neutralization; Middle East Respiratory Syndrome Coronavirus; Medicine; Female; Coronavirus Infections; Camelids, New World; CETAF-taskforce; Coronaviridae; Immunology; severe acute respiratory syndrome; Coronavirus Disease 2019 Research; Respiratory system; Article; virus shedding; Antibodies; virus antibody; virus-host; 03 medical and health sciences; blood; pathogen-host; Neutralization Tests; Virology; 616; Health Sciences; Coronavirus infection; case report; Animals; human; biotic relations; Viral Diseases in Livestock and Poultry; Biology; SARS; Corona Virus; disease model; Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); FOS: Clinical medicine; Viral shedding; temperature; Gastrointestinal Viral Infections and Vaccines Development; pathogens; Outbreak; virus load; biotic interaction; Antibodies, Neutralizing; RNA extraction; Coronavirus; Coronavirus disease 2019 (COVID-19); Disease Models, Animal; experimental infection; upper respiratory tract infection; Camelids; Disease Models; lower respiratory tract infection; Animal Science and Zoology; 2019-20 coronavirus outbreak; Middle East respiratory syndrome coronavirus

Nous avons mené un essai challenge/rechallenge dans lequel 3 alpagas ont été infectés par le coronavirus du syndrome respiratoire du Moyen-Orient. Les alpagas ont excrété le virus au challenge mais étaient réfractaires à l'excrétion supplémentaire au rechallenge au jour 21. L'essai indique que les alpagas peuvent être des modèles appropriés pour l'infection et la dynamique d'excrétion de ce virus.<br/>Realizamos un ensayo de desafío/reexposición en el que 3 alpacas se infectaron con el coronavirus del síndrome respiratorio de Oriente Medio. Las alpacas eliminaron el virus en la prueba de provocación, pero fueron refractarias a una mayor eliminación en la nueva prueba de provocación el día 21. El ensayo indica que las alpacas pueden ser modelos adecuados para la infección y la dinámica de desprendimiento de este virus.<br/>أجرينا تجربة تحدي/إعادة تحدي أصيب فيها 3 من الألبكة بفيروس كورونا المرتبط بمتلازمة الشرق الأوسط التنفسية. ألقت الألبكة الفيروس في التحدي لكنها كانت مقاومة لمزيد من الإلقاء في إعادة التحدي في اليوم 21. تشير التجربة إلى أن الألبكة قد تكون نماذج مناسبة للعدوى وديناميكيات التخلص من هذا الفيروس.<br/>We conducted a challenge/rechallenge trial in which 3 alpacas were infected with Middle East respiratory syndrome coronavirus. The alpacas shed virus at challenge but were refractory to further shedding at rechallenge on day 21. The trial indicates that alpacas may be suitable models for infection and shedding dynamics of this virus.<br/>