Human Norovirus Replication in Human Intestinal Enteroids as Model to Evaluate Virus Inactivation

• DOI: 10.3201/eid2408.180126
• Publication Date: 2018-06-27T17:53:34Z
• Authors: Veronica Costantini; Esther K. Morantz; Hannah Browne; Khalil Ettayebi; Xi-Lei Zeng; Robert L. Atmar; Mary K. Estes; Jan Vinjé
• Keywords: Murine norovirus; Infectivity; Acute gastroenteritis; Enteric virus; Virus quantification

Abstract Human noroviruses are a leading cause of epidemic and endemic acute gastroenteritis worldwide foodborne illness in the United States. Recently, human intestinal enteroids (HIEs) derived from small tissue have been shown to support norovirus replication. We implemented HIE system our laboratory tested effect chlorine alcohols on infectivity. Successful replication was observed for 6 GII genotypes dependent viral load genotype inoculum. GII.4 viruses had higher levels than other genotypes. Regardless concentration or exposure time, slightly reduced, but did not completely inactivate, norovirus. In contrast, complete inactivation 3 occurred at concentrations as low 50 ppm chlorine. Taken together, data confirm successful HIEs their utility tools study strategies.