Isolation of Legionella pneumophila by Co-culture with Local Ameba, Canada

• DOI: 10.3201/eid2511.190522
• Publication Date: 2019-10-01T16:38:36Z
• Authors: Rafik Dey; Harley Mount; Alex W. Ensminger; Greg J. Tyrrell; Linda P. Ward; Nicholas J. Ashbolt
• Keywords: Isolation; Legionella; Legionnaires' disease

Legionellosis was diagnosed in an immunocompromised 3-year-old girl Canada. We traced the source of bacterium through co-culture with ameba collected from a hot tub her home. identified Legionella pneumophila serogroup 6, sequence type 185, and used whole-genome sequencing to confirm environmental clinical isolates were common origin.