Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States

• DOI: 10.3201/eid2606.200516
• Publication Date: 2020-03-11T16:20:37Z
• Authors: Jennifer Harcourt; Azaibi Tamin; Xiaoyan Lu; Shifaq Kamili; Senthil K. Sakthivel; Janna Murray; Krista Queen; Ying Tao; Clinton R. Paden; Jing Zhang; Yan Li; Anna Uehara; Haibin Wang; Cynthia Goldsmith; Hannah A. Bullock; Lijuan Wang; Brett Whitaker; Brian Lynch; Rashi Gautam; Craig Schindewolf; Kumari G. Lokugamage; Dionna Scharton; Jessica A. Plante; Divya Mirchandani; Steven G. Widen; Krishna Narayanan; Shinji Makino; Thomas G. Ksiazek; Kenneth S. Plante; Scott C. Weaver; Stephen Lindstrom; Suxiang Tong; Vineet D. Menachery; Natalie J. Thornburg
• Keywords: Vero cell; Coronavirus; Atypical pneumonia

Abstract The etiologic agent of an outbreak pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 January 2020. A patient the United States given a diagnosis infection with this virus by state Washington and US Centers for Disease Control Prevention on 20, We isolated from nasopharyngeal oropharyngeal specimens characterized viral sequence, replication properties, cell culture tropism. found that replicates to high titer Vero-CCL81 cells Vero E6 absence trypsin. also deposited into repositories, making it broadly available public health research communities. hope open access reagent will expedite development medical countermeasures.