Retrospective Genomic Characterization of a 2017 Dengue Virus Outbreak, Burkina Faso

• DOI: 10.3201/eid2806.212491
• Publication Date: 2022-05-18T15:02:16Z
• Authors: Andrew G. Letizia; Catherine B. Pratt; Michael R. Wiley; Anne T. Fox; Mba Mosore; Bright Agbodzi; Clara Yeboah; Selassie Kumordjie; Nicholas Di Paola; Kone Cisse Assana; David Coulidiaty; Casimir Ouedraogo; Joseph H. Kofi Bo...; William Ampofo; Zékiba Tarnagda; Lassana Sangaré
• Keywords: Genetic divergence

Abstract Knowledge of contemporary genetic composition dengue virus (DENV) in Africa is lacking. By using next-generation sequencing samples from the 2017 DENV outbreak Burkina Faso, we isolated 29 genomes (5 serotype 1, 16 2 [DENV-2], and 8 3). Phylogenetic analysis demonstrated endemic nature DENV-2 Faso. We noted discordant diagnostic results, probably related to divergence between these Trioplex PCR. Forward reverse1 primers had a single mismatch when mapped genomes, explaining insensitivity molecular test. Although observed considerable homogeneity Dengvaxia TetraVax-DV-TV003 vaccine strains as well B cell epitopes compared with unique divergence. Continual surveillance needed clarify ongoing novel evolutionary dynamics circulating populations support development effective diagnostic, therapeutic, preventive countermeasures.