miR-21-5p promotes lung adenocarcinoma cell proliferation, migration and invasion via targeting WWC2

• DOI: 10.3233/cbm-201489
• Publication Date: 2020-08-11T15:35:25Z
• Authors: Guoan Wang; Yingyan Zhou; Weizhuang Chen; Yiming Yang; Jiankui Ye; Huilin Ou; Hongcheng Wu
• Keywords: Male; 0303 health sciences; Epithelial-Mesenchymal Transition; Lung Neoplasms; Computational Biology; Down-Regulation; Adenocarcinoma of Lung; Middle Aged; Up-Regulation; 3. Good health; Gene Expression Regulation, Neoplastic; MicroRNAs; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Humans; Female; Neoplasm Invasiveness; Gene Silencing; Lung; Adaptor Proteins, Signal Transducing; Cell Proliferation

OBJECTIVE: Studies have suggested that miR-21-5p and WWC2 are key players in most cancer types, yet the underlying mechanisms lung adenocarcinoma (LUAD) remain elusive. This study made in-depth research on two factors-dependent LUAD occurrence development. METHODS: Bioinformatics methods were employed to identify miRNA its target gene of interest. In all, 20 pairs tumor tissue samples matched adjacent normal along with 5 cell lines collected for evaluating aberrant expression WWC2. Dual-luciferase reporter assay was performed validate targeted relationship between A series vitro experiments including colony formation assay, EdU, wound healing Transwell conducted assessment biological behaviors. addition, Western blot carried out determine protein epithelial-mesenchymal transition (EMT)-related proteins. RESULTS: found be considerably increased cells relative human bronchial epithelial cells, whereas significantly decreased. revealed down-regulated expression. Besides, silencing or overexpressing played an inhibitory role PC-9 proliferation, migration invasion, but such effect suppressed when overexpressed. Furthermore, uncovered overexpression impeded EMT process cells. CONCLUSION: facilitates invasion through targeting WWC2, which provides a novel therapeutic treatment.