Efficient Evergreen Plant Regeneration of Cinnamomum japonicum Sieb. through in vitro Organogenesis

• DOI: 10.32604/phyton.2021.014191
• Publication Date: 2021-02-23T02:38:59Z
• Authors: Jiaji Zhang; Xiaofei Long; Yuhao Weng; Tielong Cheng; Jisen Shi; Jinhui Chen
• Keywords: Organogenesis; Gibberellic acid; Ornamental plant; Cinnamomum; Callus

Cinnamomum japonicum Sieb. is an excellent roadside tree and medicinal species with considerable ornamental economic value. In this study, we successfully developed a large-scale micropropagation protocol for C. the first time. Sterilized shoots were excised used as explants shoot induction on several basal media, supplemented different concentrations of plant growth regulators (PGRs), such Thidiazuron (TDZ), N6 -Benzyladenine (6-benzylaminopurine) (BA), α-naphthaleneacetic acid (NAA) Gibberellic (GA3). After comparison, most efficient medium regeneration was 1/2 Murashige Skoog (MS) containing 0.5 mg L–1 BA, 0.05 NAA 0.2 GA3, which resulted in average number induced per explant length 5.2 1.62 cm at 28 d, respectively. Then, elongated adventitious transferred to induce roots. 86.7% able root MS 0.1 BA. The earliest rooting time observed after 21 d up 3.3 d. Our study shows that can be regenerated through de novo organogenesis, lays foundation future transformation research tree.