Association between Clinical Characteristics and Microbiota in Bronchiectasis Patients Based on Metagenomic Next-Generation Sequencing Technology

• DOI: 10.33073/pjm-2024-007
• Publication Date: 2024-03-04T16:26:08Z
• Authors: Dongfeng Shen; Xiaodong Lv; Hui Zhang; Chunyuan Fei; Jing Feng; Jiaqi Zhou; Linfeng Cao; Ying Ying; Na Li; Xiaolong Ma
• Keywords: Microbiological culture; Blood Culture; Sputum culture

Abstract This study aimed to investigate the disparities between metagenomic next-generation sequencing (mNGS) and conventional culture results in patients with bronchiectasis. Additionally, we sought correlation clinical characteristics of their microbiome profiles. The overarching goal was enhance effective management treatment bronchiectasis patients, providing a theoretical foundation for healthcare professionals. A retrospective survey conducted on 67 admitted First Hospital Jiaxing from October 2019 March 2023. Clinical baseline information, inflammatory indicators, pathogen detection reports, including mNGS, blood culture, bronchoalveolar lavage fluid (BALF) sputum results, were collected. By comparing mNGS differences rate types explored, diagnostic performance compared evaluated. Based various pathogens detected by association microbiota analyzed. number significantly larger than those culture. efficacy superior all pathogens, particularly viral ( p < 0.01). Regarding rate, bacteria highest Pseudomonas aeruginosa (17/58) Haemophilus influenzae (11/58); fungus Aspergillus fumigatus (10/21), virus human herpes 4 (4/11). Differences observed positive negative groups P. terms common scoring systems whether main symptom manifested as thick 0.05). Significant distinctions also noted A. regarding Reiff score, neutrophil percentage, etiology, alterations plans following reporting Notably, 70% infection opted change plans. microbiological revealed that bacteria, such , fungi, associated specific features patients. underscored significance guiding personalized approaches. could identify multiple different samples rapid tool identification. Its use recommended diagnosing causes infections