Development and Evaluation of a Rapid GII Norovirus Detection Method Based on CRISPR-Cas12a

• DOI: 10.33073/pjm-2024-009
• Publication Date: 2024-03-04T16:25:38Z
• Authors: Xinyi Hu; Pei He; Tong Jiang; Jilu Shen
• Keywords: Acute gastroenteritis

Norovirus is highly infectious and rapidly transmissible represents a major pathogen of sporadic cases outbreaks acute gastroenteritis worldwide, causing substantial disease burden. Recent years have witnessed dramatic increase in norovirus China, significantly higher than previous years, among which GII the predominant prevalent strain. Therefore, rapid diagnosis critical for clinical treatment transmission control. Hence, we developed molecular assay based on RPA combined with CRISPER-CAS12a technique targeting conserved region genome, results could be displayed by fluorescence curves immunochromatographic lateral-flow test strips. The reaction only required approximately 50 min, were visible naked eye sensitivity reaching 10