Is There Additive Therapeutic Effect When GCSF Combined with Adipose-Derived Stem Cell in a Rat Model of Acute Spinal Cord Injury?

• DOI: 10.3340/jkns.2016.1010.008
• Publication Date: 2017-06-30T00:32:31Z
• Authors: Joongkee Min; Jeong Hoon Kim; Kyoung Hyo Choi; Hyung Ho Yoon; Sang Ryong Jeon
• Keywords: Stem Cell Therapy

Functional and neural tissue recovery has been reported in many animal studies conducted with stem cells. However, the combined effect of cytokines cells not yet adequately researched. Here, we analyzed additive effects granulocyte colony-stimulating factor (GCSF) on adipose-derived (ADSCs) infusion treatment acute spinal cord injury (SCI) rats.Four days after intrathecal tubes implantation Sprague-Dawley rats, SCI was induced an infinite horizon impactor. In Sham group (n=5), phosphate-buffered saline injected 3, 7, 14 SCI. GCSF, ADSCs, ADSCs GCSF were at same time (n=8), ADSC ADSC+GCSF groups (n=7), respectively.The groups, but group, showed significantly higher Basso-Beattie-Bresnahan scores than during 8 weeks (p<0.01), no significant difference between groups. ladder rung test, all four different from each other, showing best improvement (p<0.01). On immunofluorescent staining (GAP43, MAP2), western blotting (GAP43), reverse transcription polymerase chain reaction nerve growth factor), levels groups.Our analyses suggest that combination infusions rat does have a effect. Hence, when agents for cell therapy are considered, molecules other or modifications to methodology, should be investigated.