Comparison of Four Pancreatic Islet Implantation Sites

• DOI: 10.3346/jkms.2010.25.2.203
• Publication Date: 2010-01-26T06:23:00Z
• Authors: Hyoung-Il Kim; Jae Eun Yu; Chung-Gyu Park; Sang-Joon Kim
• Keywords: Blood Glucose; Transplantation, Heterotopic; Experimental/chemically induced; Islets of Langerhans Transplantation; Heterotopic*; Inbred C57BL; Kidney; Diabetes Mellitus, Experimental; Islets of Langerhans; Mice; 03 medical and health sciences; Diabetes Mellitus; 617; Animals; Muscle, Skeletal; Transplantation; 0303 health sciences; Experimental/mortality; Muscles; Skeletal; Blood Glucose/analysis; Glucose Tolerance Test; Islets of Langerhans Transplantation*; 3. Good health; Mice, Inbred C57BL; Liver; Hyperglycemia; Muscle; Original Article; Omentum; Hyperglycemia/therapy

Although the liver is the most common site for pancreatic islet transplantation, it is not optimal. We compared kidney, liver, muscle, and omentum as transplantation sites with regard to operative feasibility, and the efficiency of implantation and glycemic control. Islets from C57BL/6 mice were transplanted into diabetic syngeneic recipients. The mean operative time and mortality were measured to assess feasibility. To assess implantation efficiency, the marginal mass required to cure diabetes and the mean time taken to achieve normoglycemia were measured. A glucose tolerance test was performed to assess glycemic control efficiency. The data are listed in the order of the kidney, liver, muscle, and omentum, respectively. The mean mortality rate was 6.7, 20.0, 7.1, and 12.5%; the mean operative time was 10.2, 27.4, 11.2, and 19.8 min; the marginal islet mass was 100, 600, 600, and 200 islet equivalence units and the mean time to reach euglycemia was 3.0, 15.1, 26.6, and 13.9 days. The glucose kinetics of omental pouch islets was the most similar to controls. Thus, a strategic approach is required for deciding on the best transplantation recipient sites after considering donor sources and islet volume. Alternatives can be chosen based on safety or efficacy.