Broilers with low serum Mannose-binding Lectin show increased fecal shedding of Salmonella enterica serovar Montevideo

• DOI: 10.3382/ps/pew101
• Publication Date: 2016-03-19T01:50:12Z
• Authors: Sofie L. Ulrich-L...; Helle R. Juul-Madsen; Rikke B. Kjærup; Ron Okimoto; Mitchell S. Abrah...; Sven Maurischat; Poul Sørensen; Tina S. Dalgaard
• Keywords: Bacterial Shedding; Male; 2. Zero hunger; Salmonella Infections, Animal; 0303 health sciences; broilers; chicken; Salmonella enterica; Real-Time Polymerase Chain Reaction; Salmonella enterica subsp. enterica serovar Montevideo; Mannose-Binding Lectin; 03 medical and health sciences; Animals; mannose-binding lectin; Female; real-time PCR; Chickens; Poultry Diseases; Disease Resistance

Mannose-binding lectin (MBL) is a key molecule in innate immunity. MBL binds to carbohydrates on the surface of pathogens, initiating the complement system via the lectin-dependent pathway or facilitates opsonophagocytosis. In vivo studies using inbred chicken lines differing in MBL serum concentration indicate that chicken MBL affects Salmonella resistance; further studies are imperative in conventional broiler chickens. In this study 104 conventional day-old chickens (offspring from a cross between Cobb 500 male and female parent breeders) were orally infected with Salmonella enterica subsp. enterica serovar Montevideo. The chickens were divided into two groups based on polymorphisms in their MBL promoter region, designated L/L for low serum concentrations of MBL and L/H for medium serum concentrations of MBL. A semi-quantitative real-time PCR method for detection of Salmonella in cloacal swabs was used, the log10 CFU quantification was based on a standard curve from artificially spiked cloacal swab samples pre-incubated for 8 h with known concentrations of Salmonella ranging from 10(1) to 10(6) CFU/swabs, with an obtained amplification efficiency of 102% and a linear relationship between the log10 CFU and the threshold cycle Ct values of (R(2) = 0.99). The L/L chickens had significantly higher Log10 CFU/swab at week 5 post infection (pi) than the L/H chickens. A repetition of the study with 86 L/L and 18 L/H chickens, also gave significantly higher log10 CFU ± SEM in cloacal swabs, using the semi-quantitative real-time PCR method from L/L chickens than from the L/H chickens at week 5 pi. These results indicate that genetically determined basic levels of MBL may influence S. Montevideo susceptibility.