Protective effect of Salvia miltiorrhiza polysaccharides on liver injury in chickens

• DOI: 10.3382/ps/pez153
• Publication Date: 2019-04-05T22:00:15Z
• Authors: Chao Han; Yuanyuan Wei; Xiao Wang; Cuijing Ba; Wanyu Shi
• Keywords: Salvia miltiorrhiza; Aspartate transaminase; Malondialdehyde; CCL4

This study investigated the effects of Salvia miltiorrhiza polysaccharides (SMPs) on injury chicken hepatocytes in vitro and vivo. In studies primary cultured were isolated by 2-step collagenase perfusion. Carbon tetrachloride (CCL4) was added to establish a hepatocyte model. Hepatocytes treated with different concentrations SMPs detect protective CCL4-induced injury. The results control group showed that grew well their morphology normal. After CCL4 treatment, activity alanine transaminase (ALT) aspartate (AST) increased compared normal group. treatment downregulated contents ALT, AST, malondialdehyde (MDA), upregulated glutathione (GSH) cytochrome P450 (CYP450). An acute liver model established vivo 2.0 mL/kg 50% CCL4. Oral administration SMP at doses exhibited preventive success. group, total protein (TP), albumin (Alb), GSH significantly decreased levels index, MDA increased. contrast, TP, Alb, increased, Therefore, we conclude have good effect damage vitro.