In Ovo Administration of Salmonella enteritidis-Immune Lymphokines Confers Protection to Neonatal Chicks Against Salmonella enteritidis Organ Infectivity

• DOI: 10.3382/ps.0740018
• Publication Date: 2012-04-21T22:28:59Z
• Authors: E.D. MCGRUDER; G.A. RAMIREZ; M.H. KOGUT; R.W. MOORE; D.E. CORRIER; J.R. DELOACH; B.M. HARGIS
• Keywords: Salmonella enteritidis; In ovo; Infectivity

We previously reported that the prophylactic, intraperitoneal administration of supernatants from concanavalin A-stimulated T cells derived Salmonella enteritidis (SE)-immune White Leghorn hens (i.e., SE-immune lymphokines or ILK), conferred protection to neonatal chicks against SE organ invasion. In present study, we evaluated effects in ovo ILK on hatchability, hatch weight, vitro bactericidal activity heterophils, and invasion chicks. On Day 18 embryogenesis, injections were made into amnion with either nonimmune (NILK) not injected (untreated). day hatch, whole blood was collected 20 per treatment group for heterophil isolation. All remaining orally challenged 5 x 10(4) cfu SE. Twenty-four hours after challenge, organs (liver spleen) cultured Hatchability ILK- NILK-treated different untreated Hatch weights ILK-treated approximately 1 g less (P < .05) than peripheral heterophils increased above Organ markedly significantly decreased as compared treated NILK These results suggest confers infectivity at hatch.