Development of a Bone-Mimetic 3D Printed Ti6Al4V Scaffold to Enhance Osteoblast-Derived Extracellular Vesicles’ Therapeutic Efficacy for Bone Regeneration

• DOI: 10.3389/fbioe.2021.757220
• Publication Date: 2021-10-26T06:54:57Z
• Authors: Kenny Man; Mathieu Y. Brunet; Sophie Louth; Thomas E. Robinson; Maria Fernandez-R...; Soraya Williams; Angelica S. Federici; Owen G. Davies; David A. Hoey; Sophie C. Cox
• Keywords: Microvesicle

Extracellular Vesicles (EVs) are considered promising nanoscale therapeutics for bone regeneration. To date, EVs typically procured from cells on 2D tissue culture plastic, an artificial environment that limits cell growth and does not replicate in situ biochemical or biophysical conditions. This study investigated the potential of 3D printed titanium scaffolds coated with hydroxyapatite to promote therapeutic efficacy osteoblast-derived EVs. Ti6Al4V different pore sizes (500 1000 µm) shapes (square triangle) were fabricated by selective laser melting. A bone-mimetic nano-needle (nnHA) coating was then applied. scaffold-cultured osteoblasts over 2 weeks vesicle concentration determined using CD63 ELISA. Osteogenic differentiation human marrow stromal (hBMSCs) following treatment primed evaluated assessing alkaline phosphatase activity, collagen production calcium deposition. Triangle significantly increased osteoblast mineralisation (1.5-fold) when compared square architectures (P ≤ 0.001). Interestingly, EV yield also enhanced these higher permeability structures 0.001), particular (2.2-fold) larger (1000 µm). Furthermore isolated triangular hBMSCs acquired (1.7-fold) Coating nnHA improved (>2.6-fold) (4.5-fold) uncoated Together, findings demonstrate harnessing platforms enhance pro-regenerative as acellular tool repair.