Effect of Decellularized Extracellular Matrix Bioscaffolds Derived from Fibroblasts on Skin Wound Healing and Remodeling

• DOI: 10.3389/fbioe.2022.865545
• Publication Date: 2022-06-29T07:51:33Z
• Authors: Hyo-Sung Kim; Hyun-Jeong Hwang; Han-Jun Kim; Yeji Choi; Daehyung Lee; Hong-Hee Jung; Sun Hee Do
• Keywords: Decellularization; Human skin

The mammalian tissue extracellular matrix (ECM) has been used as a scaffold to facilitate the repair and reconstruction of numerous tissues. However, material properties decellularized ECM (dECM) from in vitro cell cultures effect these on wound remodeling remain unclear. To elucidate its biological activity, we extracted dECM human lung fibroblasts, fabricated it into patch, applied full-thickness skin wound. fibroblast-derived (fdECM) maintained content collagen Ⅰ, Ⅳ, elastin, extraction process did not damage critical growth factors. fdECM-conjugated patch (COL-fdECM) facilitated contraction angiogenesis proliferative phase when vivo model. Moreover, COL-fdECM treated showed increased regeneration epidermal barrier function, mature collagen, hair follicle, subepidermal nerve plexus, suggesting qualitative remodeling. This therapeutic efficacy was similarly observed diabetic ulcer fdECM shown help remodel by regulating fibroblast factors, metalloproteinases, inhibitors metalloproteinases via p38 ERK signaling pathways an experiment for understanding underlying mechanism. These results provide basis cell-derived multi-functional biomaterial applicable various diseases.