A simple and efficient strategy for trace detection of ferroptosis-related miRNAs based on novel hydrophobic paper-based plasmonic substrate and “inverse molecular sentinel (iMS)” nanoprobes

• DOI: 10.3389/fbioe.2023.1146111
• Publication Date: 2023-03-02T06:10:42Z
• Authors: Youwei Wang; Bing Chen; Jiang Fan; Zhong Wang
• Keywords: Surface-Enhanced Raman Spectroscopy; Nanorod

Monitoring ferroptosis-related miRNAs is crucial for the treatment and prognosis of patients with intracerebral hemorrhage. In this work, a novel hydrophobic paper (h-paper)-based plasmonic substrate was produced by dropping DS Au nanorods narrow range sizes morphologies onto h-paper. Raman reporter molecules were adsorbed to array surface, surface-enhanced scattering spectra at randomly selected points reveal uniform significant SERS enhancement. Hairpin DNAs labelled reporters hybridized placeholder decorated on fabricate biosensor. Target initiated "inverse Molecular Sentinel" process. During process, PHs removed conformation HPs changed toward hairpin structure, thus eliciting proximity stronger signal. The proposed biosensor performs well in terms stability, reproducibility, selectivity. limits detection miR-122-5p miR-140-5p serum 4.17 aM 4.49 aM, respectively. Finally, fabricated applied detect ICH healthy subjects, results obtained consistent from quantitative real-time polymerase chain reaction, revealing accuracy method. This simple, rapid approach offers great potential simultaneous practical clinical applications.