Genome-wide transcription landscape of citric acid producing Aspergillus niger in response to glucose gradient

• DOI: 10.3389/fbioe.2023.1282314
• Publication Date: 2023-10-25T06:14:45Z
• Authors: Xiaomei Zheng; Peng Du; Kaiyue Gao; Yimou Du; Timothy C. Cairns; Xiaomeng Ni; Meiling Chen; Wei Zhao; Xinrong Ma; Hongjiang Yang; Ping Zheng; Jibin Sun
• Keywords: Aspergillus niger; Catabolism; Snf3

Aspergillus niger is the main industrial workhorse for global citric acid production. This fungus has complex sensing and signaling pathways to respond environmental nutrient fluctuations. As preferred primary carbon source, glucose also acts as a critical signal trigger intracellular bioprocesses. Currently, however, there still knowledge gap in systems-level understanding of metabolic cellular responses this vital source. In study, we determined genome-wide transcriptional changes acid-producing response external gradient. It demonstrated that fluctuation led reprogramming many genes encoding proteins involved fundamental process, including ribosomal biogenesis, transport catabolism, signaling. The major catabolism repressor creA maintained stable expression independent glucose, while creB creD showed significant downregulation upregulation by increase. Notably, several high-affinity transporters genes, mstA, were greatly upregulated when was depleted, low-affinity transporter mstC glucose-independent, which clear concordance with their protein levels detected situ fluorescence labeling assay. addition, observed exporter cexA be transcriptionally regulated availability, correlated extracellular secretion. These discoveries not only deepen our regulation but shed new light on adaptive evolutionary mechanism production A. niger.