Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacterium

• DOI: 10.3389/fbioe.2023.1335854
• Publication Date: 2024-01-08T04:46:40Z
• Authors: Hossam S. El-Beltagi; Asmaa A. Halema; Zainab M. Almutairi; Hayfa Habes Almut...; Nagwa I. Elarabi; Abdelhadi A. Abde...; Ahmed R. Henawy; Heba A. R. Abdelh...
• Keywords: Genome size; Lead (geology)

Lead pollution of the environment poses a major global threat to ecosystem. Bacterial bioremediation offers promising alternative traditional methods for removing these pollutants, that are often hindered by various limitations. Our research focused on isolating lead-resistant bacteria from industrial wastewater generated heavily lead-containing industries. Eight strains were successfully isolated, and subsequently identified through molecular analysis. Among these, Enterobacter kobei FACU6 emerged as particularly candidate, demonstrating an efficient lead removal rate 83.4% remarkable absorption capacity 571.9 mg/g dry weight. Furthermore, E. displayed maximum tolerance concentration (MTC) reaching 3,000 mg/L. To further investigate morphological changes in response exposure, scanning electron microscopy (SEM) transmission (TEM) employed. These analyses revealed significant adsorption intracellular accumulation treated contrast control bacterium. Whole-genome sequencing was performed gain deeper insights into E . kobei’s resistance mechanisms. Structural annotation genome size 4,856,454 bp, with G + C content 55.06%. The encodes 4,655 coding sequences (CDS), 75 tRNA genes, 4 rRNA genes. Notably, genes associated heavy metal their corresponding regulatory elements within genome. expression levels four specific evaluated. findings statistically upregulation gene under environmental conditions, including pH 7, temperature 30°C, high concentrations metals. outstanding potential source diverse related plant growth promotion makes it valuable candidate developing safe effective strategies disposal.