Amine-reactive crosslinking enhances type 0 collagen hydrogel properties for regenerative medicine

• DOI: 10.3389/fbioe.2024.1391728
• Publication Date: 2024-07-26T04:31:54Z
• Authors: Daniel Salthouse; Peter D. Goulding; Sophie L. Reay; Emma L. Jackson; Chenlong Xu; Rochelle Ahmed; Andrew Mearns-Spragg; Katarina Novakovic; Catharien M. U. H...; Ana Marina Ferreira
• Keywords: Regenerative Medicine; Type I collagen

Introduction Collagen is extensively utilised in regenerative medicine due to its highly desirable properties. However, collagen typically derived from mammalian sources, which poses several limitations, including high cost, potential risk of immunogenicity and transmission infectious diseases, ethical religious constraints. Jellyfish-sourced type 0 represents a safer more environmentally sustainable alternative source. Methods Thus, we investigated the jellyfish collagen-based hydrogels, obtained Rhizostoma pulmo (R. pulmo) jellyfish, be medicine. A variety R. hydrogels (RpCol hydrogels) were formed by adding range chemical crosslinking agents their physicochemical biological properties characterised assess suitability for applications. Results Discussion The characteristic composition RpCol was confirmed Fourier-transform infrared spectroscopy (FTIR), degradation kinetics, morphological, rheological shown adaptable through addition specific agents. endotoxin levels below Food Drug Administration (FDA) limit medical devices, thus allowing use vivo . 8-arm polyethylene glycol succinimidyl carboxyl methyl ester (PEG-SCM)-crosslinked preserved viability induced significant increase metabolic activity immortalised human mesenchymal stem/stromal cells (TERT-hMSCs), therefore demonstrating wide