Rapid and Reproducible Differentiation of Hematopoietic and T Cell Progenitors From Pluripotent Stem Cells

• DOI: 10.3389/fcell.2020.577464
• Publication Date: 2020-10-20T05:15:00Z
• Authors: Léa Flippe; Anne Gaignerie; Céline Sérazin; Olivier Baron; Xavier Saulquin; Maria Themeli; Carole Guillonneau; Laurent David
• Keywords: Embryoid body

Cell therapy using T cells has revolutionized medical care in recent years but limitations are associated with the difficulty of genome editing cells, production a sufficient number and standardization product. Human pluripotent stem (hPSCs) can self-renew differentiate into to provide standardized homogenous product defined origin indefinite quantity, therefore they great potential alleviate therapeutic cell production. The differentiation hPSCs takes place two steps: first induction hematopoietic stem/progenitor (HSPCs), then lymphopoiesis by Notch signaling. However, from be difficult lack reproducibility. One parameter that needs better assessed is DLL1 versus DLL4 ligands pathway induce cells. In addition, culture labor-intensive not compatible GMP production, especially when cultured on feeder Thus, definition robust GMP-compatible protocol feeder-free conditions would increase accessibility off-the-shelf progenitors derived hPSCs. this article, we describe an efficient, rapid reproducible for generation (1) HSPCs embryoid bodies (EB) serum free medium systems, (2) directed hPSC-derived T-cell presence bone marrow stromal expressing Notch-ligands OP9-DLL1 OP9-DLL4.