Development and Validation of a Hypoxia-Associated Prognostic Signature Related to Osteosarcoma Metastasis and Immune Infiltration

• DOI: 10.3389/fcell.2021.633607
• Publication Date: 2021-03-18T08:03:07Z
• Authors: Yucheng Fu; Qiyuan Bao; Zhuochao Liu; Guoyu He; Junxiang Wen; Qi Liu; Yiqi Xu; Zhijian Jin; Weibin Zhang
• Keywords: Gene signature; Nomogram; Hypoxia

Background Increasing evidence has shown that hypoxia microenvironment relates to tumor initiation and progression. However, no studies focus on the application of hypoxia-associated genes in predicting osteosarcoma patients’ prognosis. This research aims identify related metastasis construct a gene signature predict Methods The differentially expressed messenger RNAs (DEmRNAs) were identified from Therapeutically Applicable Research Generate Effective Treatments (Target) database. Univariate multivariate cox regression analyses performed develop prognostic signature. Kaplan–Meier (KM) survival patients with high low risk scores conducted. nomogram was constructed validated external Gene Expression Omnibus (GEO) cohort. Single-sample set enrichment analysis (ssGSEA) conducted investigate relationships between immune infiltration Results Two genes, including decorin (DCN) prolyl 4-hydroxylase subunit alpha 1 (P4HA1), involved In training testing datasets, high-risk showed lower rates as independent factor. Receiver operating characteristic (ROC) curves demonstrated robustness Functional DEmRNAs among high- low-risk groups revealed immune-associated functions pathways significantly enriched. Furthermore, ssGSEA five cells (DCs, macrophages, neutrophils, pDCs, TIL) three features (CCR, APC co inhibition, Check-point) down-regulated group. Conclusion current study established novel osteosarcoma. It could act biomarker serve therapeutic guidance clinical applications.