The Role of miR-31-5p in the Development of Intervertebral Disc Degeneration and Its Therapeutic Potential

• DOI: 10.3389/fcell.2021.633974
• Publication Date: 2021-03-18T08:08:06Z
• Authors: Yong Zhou; Mingsi Deng; Jiqing Su; Wei Zhang; Dongbiao Liu; Zhengguang Wang
• Keywords: Intervertebral Disc; Aggrecan; ADAMTS

Intervertebral disc degeneration (IDD) refers to the abnormal response of cell-mediated progressive structural failure. In order understand molecular mechanism maintenance and destruction intervertebral disc, new IDD treatment methods are developed. Here, we first analyzed key regulators through microRNAs microarrays. Then, level miR-31-5p was evaluated by qRT-PCR. The association between Stromal cell-derived factor 1 (SDF-1)/CXCR7 axis assessed 3′-untranslated region (UTR) cloning luciferase assay. apoptosis cells under different treatments flow cytometer. cell proliferation EdU After model establishment, discs mice tail were harvested for histological radiographic evaluation in each group. Finally, protein levels SDF-1, CXCR7, ADAMTS-5, Col II, Aggrecan, MMP13 western blot. results show that is a regulator its down-regulated IDD. Overexpression facilitates nucleus pulposus proliferation, inhibits apoptosis, ECM formation, matrix degrading enzymes NP cells. SDF-1/CXCR7 direct target miR-31-5p. acts on regulating SDF-1/CXCR7. vitro experiments further verified up-regulation prevented development conclusion, overexpression can inhibit