Umbilical Cord Blood-Derived Exosomes From Very Preterm Infants With Bronchopulmonary Dysplasia Impaired Endothelial Angiogenesis: Roles of Exosomal MicroRNAs

• DOI: 10.3389/fcell.2021.637248
• Publication Date: 2021-03-25T07:34:41Z
• Authors: Xin-qi Zhong; Qin Yan; Zhuang-gui Chen; Chun-hong Jia; Xiu-hong Li; Zi-yan Liang; Jian Gu; Hui-ling Wei; Chang-yu Lian; Jing Zheng; Qi-liang Cui
• Keywords: Bronchopulmonary Dysplasia; Exosome

Premature infants have a high risk of bronchopulmonary dysplasia (BPD), which is characterized by abnormal development alveoli and pulmonary vessels. Exosomes exosomal miRNAs (EXO-miRNAs) from bronchoalveolar lavage fluid are involved in the BPD might serve as predictive biomarkers for BPD. However, roles exosomes EXO-miRNAs umbilical cord blood regulating angiogenesis yet to be elucidated. In this study, we showed that blood-derived impaired vitro. Next-generation sequencing preterm without (NBPD group) or with (BPD uncovered total 418 differentially expressed (DE) EXO-miRNAs. These DE were primarily enriched cellular function-associated pathways including PI3K/Akt angiogenesis-related signaling pathways. Among those associated pathways, reduced expression hsa-miR-103a-3p hsa-miR-185-5p exhibiting most significant reduction (14.3% 23.1% NBPD group, respectively); increased hsa-miR-200a-3p 2.64 folds group. Furthermore, overexpression normal human vein endothelial cells (HUVECs) significantly enhanced cell proliferation, tube formation, migration, whereas overexpressing inhibited these responses. This study demonstrates derived impair angiogenesis, possibly via EXO-miRNAs, contribute