Comparative Transcriptomic Analyses of Antibiotic-Treated and Normally Reared Bactrocera dorsalis Reveals a Possible Gut Self-Immunity Mechanism

• DOI: 10.3389/fcell.2021.647604
• Publication Date: 2021-09-21T05:33:21Z
• Authors: Jiajin Fu; Lingyu Zeng; Linyu Zheng; Zhenzhen Bai; Zhihong Li; Lijun Liu
• Keywords: Bactrocera Dorsalis; Pathogenic bacteria

Bactrocera dorsalis (Hendel) is a notorious agricultural pest worldwide, and its prevention control have been widely studied. Bacteria in the midgut of B. help improve host insecticide resistance environmental adaption, regulate growth development, affect male mating selection, among other functions. Insects an effective gut defense system that maintains self-immunity balance microorganisms gut, addition to stabilizing diversity symbiotic bacteria. However, detailed regulatory mechanisms governing bacteria are still unclear oriental fruit flies. In this study, was altered by feeding flies antibiotics, function predicted. Then, database intestinal transcriptome fly established analyzed using Illumina HiSeq Platform. The shifted from Gram negative positive after antibiotic feeding. Antibiotics lead reduction bacteria, particularly Gram-positive which ultimately reduced reproduction Ten immunity-related genes were differentially expressed response bacterial community changes selected for qRT-PCR validation. Peptidoglycan-recognition protein SC2 gene ( PGRP-SC2) one 10 analyzed. differential expression PGRP-SC2 most significant, confirms may immunity toward