Smyd1 Orchestrates Early Heart Development Through Positive and Negative Gene Regulation

• DOI: 10.3389/fcell.2021.654682
• Publication Date: 2021-04-01T06:06:44Z
• Authors: Zhen Wang; Robert J. Schwartz; Jing Liu; Fei Sun; Qi Li; Yanlin Ma
• Keywords: Chromatin immunoprecipitation; Trichostatin A; GATA4

SET and MYND domain-containing protein 1 (Smyd1) is a striated muscle-specific histone methyltransferase. Our previous work demonstrated that deletion of Smyd1 in either cardiomyocytes or the outflow tract (OFT) resulted embryonic lethality at E9.5, with cardiac structural defects such as truncation OFT right ventricle impaired expansion proliferation second heart field (SHF). The phenotype was accompanied by downregulation ISL LIM Homeobox (Isl1) upregulation atrial natriuretic factor (ANF). However, mechanisms regulating Isl1 ANF during development remain to be elucidated. Here, we employed various biochemical molecular biological approaches including chromatin immunoprecipitation polymerase chain reaction (ChIP-PCR), pGL3 fluorescence reporter system, co-immunoprecipitation (CoIP) found interacted absent small homeotic-2-like (ASH2L) activated promoter trimethylating H3K4. We also associated HDAC repress expression using trichostatin A (TSA), deacetylase inhibitor. In conclusion, participates early upregulating downregulating ANF.