Melatonin Regulates the Neurotransmitter Secretion Disorder Induced by Caffeine Through the Microbiota-Gut-Brain Axis in Zebrafish (Danio rerio)

• DOI: 10.3389/fcell.2021.678190
• Publication Date: 2021-05-20T05:38:35Z
• Authors: Zeng Zhang; Qiannan Peng; Dongxue Huo; Shuaiming Jiang; Chenchen Ma; Haibo Chang; Kaining Chen; Congfa Li; Yonggui Pan; Jiachao Zhang
• Keywords: Danio; Gut–brain axis

Melatonin has been widely used as a “probiotic agent” capable of producing strong neurotransmitter secretion regulatory effects, and the microbiota-gut-brain axis-related studies have also highlighted role gut microbiota in neuromodulation. In present study, zebrafish neural hyperactivity model was established using caffeine induction to explore effects melatonin probiotic on disorder zebrafish. Disorders brain (dopamine, γ-aminobutyric acid, 5-hydroxytryptamine) caused by were improved after interference treatment with or probiotic. Shotgun metagenomic sequencing demonstrated that melatonin-treated gradually restored their normal intestinal metabolic pathways. Germ-free (GF) verify essential microbes regulation secretion. The results short-chain fatty acid determination revealed effect GF group could not achieve adding same dose melatonin. research potential mode action through axis regulate disruption secretion, supporting future development psychotropic drugs targeting microbiota.