Digitally Driven Aerosol Jet Printing to Enable Customisable Neuronal Guidance

• DOI: 10.3389/fcell.2021.722294
• Publication Date: 2021-09-03T16:35:47Z
• Authors: Andrew J. Capel; Matthew A. A. Smith; Silvia Taccola; Maria Pardo-Figuerez; Rowan P. Rimington; Mark P. Lewis; Steven D. R. Chri...; Robert W. Kay; Russell A. Harris
• Keywords: Neural tissue engineering

Digitally driven manufacturing technologies such as aerosol jet printing (AJP) can make a significant contribution to enabling new capabilities in the field of tissue engineering disease modeling and drug screening. AJP is an emerging non-contact mask-less process which has distinct advantages over other patterning it offers versatile, high-resolution, direct-write deposition variety materials on planar non-planar surfaces. This research demonstrates ability print digitally controlled patterns that influence neuronal guidance. These consist patterned poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) tracks both glass poly(potassium 3-sulfopropyl methacrylate) (PKSPMA) coated surfaces, promoting selective adhesion SH-SY5Y neuroblastoma cells. The cell attractive had maximum height ≥0.2 μm, width half ≥15 Ra = 3.5 nm, RMS 4.1. developed biocompatible PEDOT:PSS ink was shown promote adhesion, growth differentiation cells cultured directly onto these features exhibited increased nuclei alignment substrates. In addition, substrate when PKSPMA surfaces resulting highly organized neural pattern. demonstrated rapidly flexibly realize intricate accurate by computer process.