Human Amniotic Epithelial Cells and Their Derived Exosomes Protect Against Cisplatin-Induced Acute Kidney Injury Without Compromising Its Antitumor Activity in Mice

• DOI: 10.3389/fcell.2021.752053
• Publication Date: 2022-02-03T09:12:24Z
• Authors: Xin Kang; Ying Chen; Xiaohong Xin; Menghan Liu; Yuan Ma; Yifei Ren; Jing Ji; Qi Yu; Lei Qu; Suxia Wang; Gang Liu; Chengang Xiang; Li Yang
• Keywords: Nephrotoxicity

Background: Cisplatin is a widely used chemotherapeutic drug, whereas the clinical application greatly limited by its nephrotoxic side effect. Currently, there has been no effective treatment to prevent cisplatin-induced acute kidney injury (cisplatin-AKI). Human amniotic epithelial cells (hAECs) and their derived exosomes (EXOs) have proven effectively protect against ischemia reperfusion-induced AKI, yet roles in cisplatin-AKI are still unknown. Methods: C57BL/6J mice were given two doses of cisplatin at 20 or 15 mg/kg body weight induce AKI with without mortality. hAECs EXOs injected via tail vein 1 day after administration. Serum tissues collected on fourth explore nephro-protective effects cisplatin-AKI. Lung cancer xenograft model was built subcutaneous injection A549 into BALB/c nude evaluate effect chemotherapy. Results: nephrotoxicity significantly attenuated as evidenced reduced mortality rate decreased serum creatinine (sCr) tubular score. exerted suppression TNF-α/MAPK caspase signaling pathways. In lung mouse model, administration did not promote tumor growth compromise therapeutic tumors. Conclusion: This study first demonstrate that vivo. Importantly, neither nor antitumor activity cisplatin. These results potentially support use nephro-protectors clinically.