The Role of Bmp- and Fgf Signaling Modulating Mouse Proepicardium Cell Fate

• DOI: 10.3389/fcell.2021.757781
• Publication Date: 2022-01-04T06:04:08Z
• Authors: Carlos Garcia-Pad...; Francisco Hernand...; Estefania Lozano-...; Angel Dueñas; Maria del Mar Muñ...; Isabel S. Garcia-...; Lledó Palencia-Vi...; Amelia Aranega; Diego Franco
• Keywords: BMPR2; Cell fate determination

Bmp and Fgf signaling are widely involved in multiple aspects of embryonic development. More recently non coding RNAs, such as microRNAs have also been reported to play essential roles during We previously demonstrated that microRNAs, i.e., miR-130, an role modulating early stages cardiomyogenesis. recently, we capable cell fate decision proepicardial/septum transversum (PE/ST) development, since over-expression miR-23 blocked while miR-125, miR-146, miR-223 miR-195 enhanced PE/ST-derived cardiomyogenesis, respectively. Importantly, regulation these is distinct modulated by Bmp2 Fgf2 administration chicken. In this study, aim dissect the functional mouse PE/ST their implication regulating post-transcriptional modulators impact on lineage determination. Mouse explants epicardial/endocardial cultures were distinctly administrated family members. qPCR analyses cardiomyogenic, fibrogenic differentiation markers well key elements directly epithelial mesenchymal transition evaluated. Our data demonstrate neither Bmp2/Bmp4 nor Fgf2/Fgf8 inducing fibrogenesis or EMT explants, yet deregulation several observed, contrast previous findings chicken PE/ST. RNAseq epicardium identified novel members might be differences, however, induction cardiomyogenic and/or limited. Thus our support notion species-specific differences commitment.