Lung Cancer Induces NK Cell Contractility and Cytotoxicity Through Transcription Factor Nuclear Localization

• DOI: 10.3389/fcell.2022.871326
• Publication Date: 2022-05-16T08:51:55Z
• Authors: Darren Chen Pei Wong; E Hui Clarissa Lee; Junzhi Er; Ivan Yow; Ricky Abdi Gunawa...; Owen Ang; Jingwei Xiao; Boon Chuan Low; Jeak Ling Ding
• Keywords: Contractility

Actomyosin-mediated cellular contractility is highly conserved for mechanotransduction and signalling. While this phenomenon has been observed in adherent cell models, whether/how contractile forces regulate the function of suspension cells like natural killer (NK) during cancer surveillance, unknown. Here, we demonstrated coculture settings that evolutionarily NK transcription factor, Eomes, undergoes nuclear shuttling lung surveillance. Biophysical biochemical analyses revealed mechanistic enhancement actomyosin-mediated contractility, which associated with flattening, thus enabling entry Eomes enhanced cytotoxicity. We found responded to presumed immunosuppressive TGFβ NK-lung medium sustain its intracellular through myosin light chain phosphorylation, thereby promoting localization. Therefore, our results demonstrate provoke as an early phase activation mechanism a plausible mechano-responsive protein increased There scope strategic application modulating drugs