Fetal Lung-Derived Exosomes in Term Labor Amniotic Fluid Induce Amniotic Membrane Senescence

• DOI: 10.3389/fcell.2022.889861
• Publication Date: 2022-07-04T04:49:57Z
• Authors: Shuting Wan; Pengzheng Chen; Mengqi Gu; Jing Liu; Qian Zhou; Fengyuan Zhang; Yuan Lu; Lei Li; Xietong Wang
• Keywords: Senescence; Fetal membrane

The mechanism of parturition is still unclear. Evidence has shown that delivery associated with cellular senescence the amniotic membrane. We isolated fetal lung-associated exosomes from fluid term labor (TL-exos) and verified can cause primary human epithelial cell (hAEC) apoptosis release higher levels senescence-associated secretory phenotype (SASP)-related molecules proinflammatory damage-associated molecular patterns (DAMPs) than not in (TNIL-exos). lung carcinoma lines (A549) be used as an alternative to alveolar type 2 cells producing pulmonary surfactant. Therefore, we A549 cell-derived (A549-exos) found they trigger hAEC undergo same aging process. Finally, animal experiments suggested A549-exos induced vaginal bleeding preterm pregnant mice. conclude derived lungs induce membrane senescence, which may provide new insight into delivery.