Effects of aging on the biomechanical properties of the lung extracellular matrix: dependence on tissular stretch

• DOI: 10.3389/fcell.2024.1381470
• Publication Date: 2024-04-05T04:17:46Z
• Authors: Anna Ulldemolins; Maria Narciso; Héctor Sanz-Fraile; Jorge Otero; Ramon Farré; Núria Gavara; Isaac Almendros
• Keywords: Decellularization

Introduction: Aging induces functional and structural changes in the lung, characterized by a decline elasticity diminished pulmonary remodeling regenerative capacity. Emerging evidence suggests that most biomechanical alterations lung result from composition of extracellular matrix (ECM), potentially modulating behavior cells increasing susceptibility to chronic diseases. Therefore, it is crucial investigate mechanical properties aged lung. This study aims assess ECM due aging at both residual (RV) (FV) volumes evaluate their effects on survival proliferation mesenchymal stromal (MSCs). Methods: The lungs young (4-6-month-old) (20-24-month-old) mice were inflated with optimal cutting temperature compound reach FV or non-inflated (RV). proteins laminin, collagen I fibronectin quantified immunofluorescence decellularized sections assessed using atomic force microscopy. To whether and/or RV affects MSCs, viability evaluated after 72 h. Results: Laminin presence was significantly reduced compared mice, while increased mice. In conditions, acellular softer than By contrast, becomes stiffer revealing strain hardening depends aging. Results MSCs recellularization showed similar rate all conditions. Discussion: data strongly measurements, especially models, should be carried out physiomimetic conditions rather following conventional approach. use scaffolds other disease murine/human models will help better understand potential role mechanotransduction progression diseases, regeneration cancer.