ZebraReg—a novel platform for discovering regulators of cardiac regeneration using zebrafish

• DOI: 10.3389/fcell.2024.1384423
• Publication Date: 2024-05-10T04:40:14Z
• Authors: Kateřina Apolínová; Ferran Arqué Pérez; Sylvia Dyballa; Benedetta Coppe; Nadia Mercader Huber; Javier Terriente; Vincenzo Di Donato
• Keywords: Regenerative Medicine

Cardiovascular disease is the leading cause of death worldwide with myocardial infarction being most prevalent. Currently, no cure available to either prevent or revert massive cardiomyocytes that occurs after a infarction. Adult mammalian hearts display limited regeneration capacity, but it insufficient allow complete recovery. In contrast, injured zebrafish heart muscle regenerates efficiently through robust proliferation pre-existing cells. Thus, allows its exploitation for studying genetic programs behind cardiac regeneration, which may be present, albeit dormant, in adult human heart. To this end, we have established ZebraReg, novel and versatile automated platform kinetics specific ablation larvae. combination imaging, can integrated pharmacological approaches used medium-throughput screening presumed modulators regeneration. We demonstrate versatility by identifying both anti- pro-regenerative effects genes drugs. conclusion, present tool utilised streamline process target validation gene regulators discovery new drug therapies regenerate