Gene expression changes in blastocyst hatching affect embryo implantation success in mice

• DOI: 10.3389/fcell.2025.1496298
• Publication Date: 2025-02-06T07:13:45Z
• Authors: Liyou An; Liang Zhang; Yulin Wu; Yadi Teng; Zihan Liu; Meixiang Ma; Miaolong Li; Xinrong Peng; Chenxi Liu
• Keywords: Affect

In mammalian embryonic development, blastocyst hatching is essential for normal implantation and development of the fetus. We reported previously that blastocysts out zona pellucida (ZP) exhibited site preferences were associated with pregnancy outcomes. To characterize these differences, we analyzed transcriptomes in following developing mouse within 16 h hatching: expanding (E), from A-site (A), B-site (B), C-site (C), hatched (H), non-hatching (N). By principal component analysis hierarchical cluster analysis, determined gene expression profiles A B blastocysts, which resulted good fertility, clustered closely. C N poor closely, but distantly B. Embryos at B- vs. C-sites, pregnancy, showed 178 differentially expressed genes (DEGs), mainly involved immunity, correlated positively birth rate. These DEGs primarily regulated by transcription factors TCF24 DLX3. During hatching, immune-related regulated, such as Ptgs1, Lyz2, Il-α, Cfb (upregulated) Cd36 (downregulated). immunofluorescence staining, found C3 IL-1β on extra-luminal surface trophectoderm blastocyst, suggesting they play a role maternal-fetal interactions. As developed to fully state, 307 either upregulated factor ATOH8 or downregulated SPIC switch immune pathways. Based outcome, identified three patterns complex changes transcriptional regulation network failed successfully blastocysts. LASSO regression-based model using Cd36, Cfb, Cyp17a1 predict success. This study revealed diverse, multidimensional developmental fates during short-term indicated properties embryo had major effect suggest their preimplantation affect implantation. contributes our understanding