Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model

• DOI: 10.3389/fcell.2025.1550220
• Publication Date: 2025-03-06T06:52:12Z
• Authors: Wenyu Zhuo; Mingyang Zhang; Jiajia Tan; Yang Gao; Yan Wang; Nana Wang; Jin Ma; Jiaying Zhang; Zhiheng Liu; Haitao Lv; Ying Liu

Kawasaki disease (KD) is a medium-vessel vasculitis predominantly affecting children under 5 years of age and may involve the coronary arteries. A mouse KD model was induced by Candida albicans cell wall extracts (CAWS), cardiac tissues were analyzed through integrated lactylomic proteomic profiling. The lysine lactylation (Kla) results normalized to data. Elevated serum lactate dehydrogenase (LDH) levels observed in patients. Given lactate's role as substrate for Kla, this study investigated Kla modifications KD. Proteomic analysis identified 150 upregulated proteins 18 downregulated proteins, with 38.1% located cytoplasm significant enrichment immune-related pathways. After normalization, 41 sites 37 found be data, no sites. Approximately 67.57% altered localized mitochondria. Bioinformatics indicated alterations aerobic respiration, energy production conversion, key immune- metabolism-related This enhances understanding development inform targeted therapies its prevention improved prognosis.