Expression Profiles and Potential Functions of Long Non-Coding RNAs in the Heart of Mice With Coxsackie B3 Virus-Induced Myocarditis

• DOI: 10.3389/fcimb.2021.704919
• Publication Date: 2021-08-24T16:13:57Z
• Authors: Xiang Nie; Huihui Li; Jin Wang; Yuanyuan Cai; Jiahui Fan; Beibei Dai; Chen Chen; Dao Wen Wang
• Keywords: Viral Myocarditis; Fulminant

Aims Long non-coding RNAs (lncRNAs) are critical regulators of viral infection and inflammatory responses. However, the roles lncRNAs in acute myocarditis (AM), especially fulminant (FM), remain unclear. Methods FM non-fulminant (NFM) were induced by coxsackie B3 virus (CVB3) different mouse strains. Then, expression profiles heart tissues detected sequencing. Finally, patterns analyzed Pearson/Spearman rank correlation, Kyoto Encyclopedia Genes Genomes, Cytoscape 3.7. Results First, 1,216, 983, 1,606, 2,459 differentially expressed identified CVB3-treated A/J, C57BL/6, BALB/c, C3H mice with myocarditis, respectively. Among them, 88 commonly dysregulated all four models. Quantitative real-time polymerase chain reaction analyses further confirmed that out top six upregulated Moreover, levels ENSMUST00000188819, ENSMUST00000199139, ENSMUST00000222401 significantly elevated spleen correlated severity cardiac infiltration. Meanwhile, 923 FM-specific detected, among which MSTRG.26098.49, MSTRG.31307.11, MSTRG.31357.2, MSTRG.32881.28 highly LVEF. Conclusion Expression is may play progression AM.