Rapid Detection and Differentiating of the Predominant Salmonella Serovars in Chicken Farm by TaqMan Multiplex Real-Time PCR Assay

• DOI: 10.3389/fcimb.2021.759965
• Publication Date: 2021-09-29T10:44:42Z
• Authors: Suhua Xin; Hong Zhu; Chenglin Tao; Beibei Zhang; Lan Yao; Yaodong Zhang; Dossêh Jean Apôtr...; Tao Li; Mingxing Tian; Jingjing Qi; Chan Ding; Shengqing Yu; Shaohui Wang
• Keywords: TaqMan; Multiplex; Salmonella enteritidis

Salmonella has been known as an important zoonotic pathogen that can cause a variety of diseases in both animals and humans. Poultry are the main reservoir for serovars Pullorum ( S . Pullorum), Gallinarum Gallinarum), Enteritidis Enteritidis), Typhimurium Typhimurium). The conventional serotyping methods differentiating complicated, time-consuming, laborious, expensive; therefore, rapid accurate molecular diagnostic needed effective detection prevention contamination. This study developed evaluated TaqMan multiplex real-time PCR assay simultaneous differentiation Pullorum, Gallinarum, Enteritidis, S. Typhimurium. In results, optimized was highly specific reliable all four target genes. analytical sensitivity corresponded to three colony-forming units (CFUs) these serovars, respectively. limit artificially contaminated samples 500 CFU/g without enrichment, while 10 after pre-enrichment. Moreover, applied poultry clinical samples, which achieved comparable results traditional bacteriological examination. Taken together, indicated will be promising tool diagnostics epidemiologic chicken farm products.